Use of Nonspecific, Glutamic Acid-Free, Media and High Glycerol or High Amylase as Inducing Parameters for Screening Bacillus Isolates Having High Yield of Polyglutamic Acid
Effect of glycerol on PGA yield by Bacillus licheniformis T. glycerol concentration in medium was varied from 0 to 5% (G0–G5) keeping citrate as 2% (C2) in the Bushnell and Hass medium base (pH 7). For solid medium 25 g agar/l was added. Media were inoculated using the same concentration of inoculums. Polymer yield is in g/l.