International Scholarly Research Notices / 2014 / Article / Fig 4

Research Article

Use of Nonspecific, Glutamic Acid-Free, Media and High Glycerol or High Amylase as Inducing Parameters for Screening Bacillus Isolates Having High Yield of Polyglutamic Acid

Figure 4

Effect of inoculum on PGA production by Bacillus licheniformis T. Inoculum was developed in Luria broth: cells were collected by centrifugation and concentrated from respective volume (10, 30, and 50 mL) by centrifugation, supernatant was discarded and only pellet was used to inoculate production medium (20 g sodium citrate/l, 40 g glycerol/l in Bushnell and Hass medium base pH 7).