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References

  1. B. Li, L. Fang, S. Liu, Y. Jiang, H. Chen, and S. Xiao, “The genomic and pathogenic characteristics of the highly pathogenic porcine reproductive and respiratory syndrome virus isolate WUH2,” ISRN Virology, vol. 2014, Article ID 624535, 15 pages, 2014.
ISRN Virology
Volume 2014, Article ID 624535, 15 pages
http://dx.doi.org/10.1155/2014/624535
Research Article

The Genomic and Pathogenic Characteristics of the Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus Isolate WUH2

1Division of Animal Infectious Diseases, State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China
2Key Laboratory of Veterinary Biological Engineering and Technology, Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Ministry of Agriculture, Jiangsu Province, Nanjing 210014, China
3Laboratory of Animal Virology, College of Veterinary Medicine, Huazhong Agricultural University, 1 Shi-zi-shan Street, Wuhan 430070, China

Received 18 November 2013; Accepted 24 December 2013; Published 23 February 2014

Academic Editors: L. Darwich and D. J. Jackwood

Copyright © 2014 Bin Li et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

To fully understand the extent of genetic diversity and pathogenesis of the highly pathogenic PRRSV found in China, we determined the genomic sequence of PRRSV WUH2; the pathogenicity of WUH2 was compared to the classical PRRSV isolate CH-1a. Our results showed that the WUH2 genome had a discontinuous deletion of 30 aa in Nsp2, a 1 nucleotide deletion located in both the 5′ and 3′ UTRs, and point mutations within GP5. Experimental infection demonstrated that PRRSV WUH2 reproduced the phenotype and symptoms of porcine high fever syndrome. Importantly, we found that there were differences in viral burden in the serum and tissues when comparing infections of the pathogenic isolate WUH2 to those of the classical isolate CH-1a. These data provide insight into the genomic diversity and altered pathogenicity of Chinese PRRSV isolates and help elucidate the evolution and potential pathogenic mechanisms of PRRSV.