Rapid Detection of Rifampicin and Isoniazid Resistant Mycobacterium tuberculosis Using Genotype MTBDRplus Assay in Nepal

Table 6

Multiple mutations observed using Genotype MTBDRplus assay.

LPA probes	Mutation sites	Mutation detected	Number of isolates (%)
rpoB			All RIF^risolates ()
WT 3, 4, MUT1	513–518	D516V	5 (14.7)
WT8, MUT 3	530–533	S531L	16 (47.1)
WT 8, MUT1	531, 533	D516V	1 (1.9)
WT 1, 6, 7, 8	505–509, 518–533		1 (1.9)
WT 6, 7, 8	518–533		1 (1.9)
WT 3, 4	513–518		1 (1.9)
WT 2, 3	510–516		1 (1.9)
katG			All INH^risolates ()
WT, MUT1	315	315 (S315T1)	29 (90.6)
WT, MUT 2	315	315 (S315T2)	1 (3.1)
inhA			All INH^risolates ()
WT1, MUT1	−15, −16	C15T	1 (3.1)