Table of Contents
ISRN Physiology
Volume 2014, Article ID 676235, 7 pages
Clinical Study

Effects of Nitrate Supplementation on Cardiovascular and Autonomic Reactivity in African-American Females

1Department of Health, Human Performance and Leisure Studies, and the Cancer Center Physical Medicine and Nutrition Laboratory, Howard University, Washington, DC 20059, USA
2Division of Cardiology, Department of Internal Medicine, Howard University College of Medicine and Howard University Hospital, Washington, DC 20060, USA
3Department of Neurology, Howard University College of Medicine and Howard University Hospital, Washington, DC 20060, USA
4Department of Physics and Astronomy, Howard University, Washington, DC 20059, USA
5Department of Physiology & Biophysics, Howard University College of Medicine, Washington, DC 20059, USA

Received 21 November 2013; Accepted 11 January 2014; Published 23 February 2014

Academic Editors: A. Kamiya, S. Perrey, and A. Tse

Copyright © 2014 Vernon Bond et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Previous studies have shown that beetroot juice (BJ) decreases systolic blood pressure (SBP) and oxygen demand. This study tests the hypothesis that a beetroot juice (BJ) treatment increases heart rate variability (HRV) measured by the average standard deviation of normal-normal electrocardiogram RR intervals (SDNN) and the low frequency (LF), mainly sympathetic, fast Fourier transform spectral index of HRV. The subjects were 13 healthy young adult African-American females. Placebo control orange juice (OJ) and BJ treatments were given on separate days. Blood nitric oxide [NO], SBP and RR intervals were measured at rest and at constant workloads set to 40% and 80% of the predetermined . Two hours after ingestion the BJ treatment increased [NO] and decreased SBP. BJ also increased SDNN at rest and at the 40% workload, without significant effects on LF. SDNN was significantly greater after the BJ than after the OJ treatment, across the two physical activity conditions and SDNN was (negatively) correlated with SBP. These results suggest that BJ decreases SBP and increases HRV at rest and during aerobic exercise. Similar results in subjects with prehypertension or hypertension could translate to a dietary nitrate treatment for hypertension.