Research Article

Modulation of Arachidonic Acid Metabolism in the Rat Kidney by Sulforaphane: Implications for Regulation of Blood Pressure

Figure 3

HPLC analysis of AA metabolism by rat kidney microsomes. (a) Representative chromatogram after incubation of AA with kidney microsomes from SHRs treated with 20 mg/kg SF in drinking water for 8 weeks. (b) Representative chromatogram produced after incubation of AA with untreated Sprague-Dawley rat kidney microsomes in the absence of NADPH. Additional details of the reactions and chromatographic conditions are described under Section 2.