Actin filaments in a chicken primary culture. In this laser confocal image, actin filaments are stained with red phalloidin, paxillin with green antibodies, and nuclei with Dapi. A single large myotube is seen on the right, while mononucleated fibroblasts and/or myoblasts appear on the left. While the more cortical actin filaments terminate in paxillin adhesion regions around which the mononucleated cells attach, several striated myofibrils run along the myotube, and only a single, large paxillin region can be seen. The myotube nuclei are out of focus and thus not visible in this single confocal slice.
