Elucidation of Underlying Mechanisms by Which Millettia macrophylla Benth Induces Its Estrogenic Activity
Figure 1
Effects of DCM and MeOH extracts on the activation of estrogen and receptors in HEK293T cells in the absence or in the presence of ICI 182,780 at 500 nM. The effect of DCM and MeOH extracts on estrogen and receptors activity in the transiently transfected HEK293T-ER and HEK293T-ER cells was investigated by measuring reporter gene-coupled luciferase activity. The relative luciferase units (RLU) were measured in the presence of DMSO (0.1%), E2 (10 nM), DCM, and MeOH extracts. , , and compared with control; , , and compared with the same dose of each substance without ICI 182,780.
