International Scholarly Research Notices / 2014 / Article / Fig 5

Research Article

Preventive Effects of a Kampo Medicine, Kakkonto, on Inflammatory Responses via the Suppression of Extracellular Signal-Regulated Kinase Phosphorylation in Lipopolysaccharide-Treated Human Gingival Fibroblasts

Figure 5

Effects of kakkonto on LPS-induced ERK phosphorylation. HGFs were untreated (0 h), treated with LPS (10 ng/mL), or treated with both LPS and kakkonto (1 mg/mL) for 0.5, 1, and 2 h. As a positive control, HGFs were treated with 1 μM of PMA for 0.5 h. Western blotting was performed using antiphosphorylated ERK or anti-ERK antibodies. pERK: phosphorylated ERK. Upper band indicates ERK1 (p44 MAPK) and lower band indicates ERK2 (p42 MAPK).

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