International Scholarly Research Notices / 2014 / Article / Tab 3

Research Article

Comparison of Reverse Transcription Quantitative Real-Time PCR, Flow Cytometry, and Immunohistochemistry for Detection of Monoclonality in Lymphomas

Table 3

RT-qPCR data for 5 B-lymphoid tumor cell lines.

CellinjeKappa CqLambda CqKappa noRT CqLambda noRT CqmRNA expressionIHC

U698 10.313.82723.5Kappa > LambdaKappa
DG7510.415.925.224.2Kappa > LambdaKappa
MC11634.810.4Not detected23.5Lambda onlyLambda
RPMI822612.214.425.122.2Kappa > LambdaLambda
CCRF-SB12.311.226.724.4Lambda </ KappaKappa
Neg. control>33~30

Cq: cycle of quantification (log transcript numbers); noRT: samples without reverse transcriptase; IHC: immunohistochemistry.

Article of the Year Award: Outstanding research contributions of 2020, as selected by our Chief Editors. Read the winning articles.