Table of Contents
ISRN Corrosion
Volume 2014, Article ID 803219, 11 pages
Research Article

Characterization of Corrosive Bacterial Consortia Isolated from Water in a Cooling Tower

1Department of Chemical & Biomolecular Engineering, National University of Singapore, Singapore 117576
2Environmental Molecular Microbiology Lab, Department of Biotechnology, Thiruvalluvar University, Serkkadu, Vellore 632 115, India

Received 16 October 2013; Accepted 13 November 2013; Published 4 March 2014

Academic Editors: M. Criado, W. Fürbeth, S. J. Lee, and Q. Qu

Copyright © 2014 Rajasekar Aruliah and Yen-Peng Ting. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


An analysis of a culturable corrosive bacterial community in water samples from a cooling tower was performed using traditional cultivation techniques and its identification based on 16S rRNA gene sequence. Seven aerobic bacterial species were identified: Pseudomonas putida ARTYP1, Pseudomonas aeruginosa ARTYP2, Massilia timonae ARTYP3, Massilia albidiflava ARTYP4, Pseudomonas mosselii ARTYP5, Massilia sp. ARTYP6, and Pseudomonas sp. ARTYP7. Although some of these species have commonly been observed and reported in biocorrosion studies, the genus Massilia is identified for the first time in water from a cooling tower. The biocorrosion behaviour of copper metal by the new species Massilia timonae ARTYP3 was selected for further investigation using a weight loss method, as well as electrochemical and surface analysis techniques (SEM, AFM, and FTIR). In contrast with an uninoculated system, thin bacterial biofilms and pitting corrosion were observed on the copper metal surface in the presence of M. timonae. The use of a biocide, bronopol, inhibited the formation of biofilm and pitting corrosion on the copper metal surface.