Table of Contents
International Scholarly Research Notices
Volume 2014, Article ID 817102, 9 pages
http://dx.doi.org/10.1155/2014/817102
Research Article

Relationship between Metabolic Fluxes and Sequence-Derived Properties of Enzymes

Institute of Microbiology and Biotechnology, University of Latvia, Kronvalda Boulevard 4, Riga LV-1010, Latvia

Received 16 April 2014; Accepted 24 August 2014; Published 29 October 2014

Academic Editor: Fernando Tadeo

Copyright © 2014 Peteris Zikmanis and Inara Kampenusa. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Metabolic fluxes are key parameters of metabolic pathways being closely related to the kinetic properties of enzymes, thereby could be dependent on. This study examines possible relationships between the metabolic fluxes and the physical-chemical/structural features of enzymes from the yeast Saccharomyces cerevisiae glycolysis pathway. Metabolic fluxes were quantified by the COPASI tool using the kinetic models of Hynne and Teusink at varied concentrations of external glucose. The enzyme sequences were taken from the UniProtKB and the average amino acid (AA) properties were computed using the set of Georgiev’s uncorrelated scales that satisfy the VARIMAX criterion and specific AA indices that show the highest correlations with those. Multiple linear regressions (88.41% ; ) were found between the values of metabolic fluxes and the selected sets of the average AA properties. The hydrophobicity, α-helicity, and net charge were pointed out as the most influential characteristics of the sequences. The results provide an evidence that metabolic fluxes of the yeast glycolysis pathway are closely related to certain physical-chemical properties of relevant enzymes and support the view on the interdependence of catalytic, binding, and structural AA residues to ensure the efficiency of biocatalysts and, hence, physiologically adequate metabolic processes.