Development of SCAR Primers for PCR Assay to Detect Diplodia seriata
Figure 1
Random amplified polymorphic DNA of Diplodia seriata using primer OPE-20: amplified fragments were separated by 1.5% agarose gel electrophoresis (TAE). Lanes 1 to 17 corresponding, respectively, to D. seriata isolates: V14-2a, Y79-4-3a, Y103-3-3b, CBS719.85, Y84-1-1a, Y221-14-3a, Y213-8-2c, Napa-c, CBS112555, Y62-1-1c, Y90-10-1a, Y46-1-1a, Y112-24-1a, Y128-3-1b, Y168-21-1b, Y181-13-1b, and Y178-11-1a; lane 18: D. mutila Y50-7-2b; and lane 19: H2O. Lane M: DNA molecular weight marker; the fragment size is indicated on the right. RAPD marker OPE-1200 bp is indicated by an arrow.
