Development of SCAR Primers for PCR Assay to Detect Diplodia seriata
Figure 2
SCAR primers specificity. The amplified fragments were separated by 1.5% agarose gel electrophoresis (TBE). (a) The PCR amplifications were carried out using the primer pair DS3.8 S3-DS3.8 R4 yielding 233 bp amplicon. Lane 1: DNA molecular weight marker; the fragment size is indicated on the right. Lanes 2 to 10 corresponding, respectively, to D. seriata isolates: CBS719.85c, R21-1a, Y46-8-1b, Y63-4-1b, Y87-3-1c, Y111-27-1, Y116-27-96, Y121-15-4, and Y125-12-1b. Lanes 11 to 19 corresponding, respectively, to controls DNA of others species: D. mutila Y128-10-1, N. parvum Y159-24-1, B. dothidea CBS110302, L. theobromae CBS110.11, D. sarmentorum CBS120.41, D. iberica Y81-1-2, N. luteum CBS110299, P. chlamydospora CBS101359, and P. aleophilum CBS631.94b. (b) DS3.8 S3-DS3.8 R6 yielding 634 bp fragment. Lanes 1 to 9 corresponding, respectively, to controls DNA of others species: D. mutila Y128-10-1, N. parvum Y159-24-1, B. dothidea CBS110302, L. theobromae CBS110.11, D. sarmentorum CBS120.41, D. iberica Y81-1-2, N. luteum CBS110299, P. chlamydospora CBS101359, and P. aleophilum CBS631.94b. Lanes 10 to 18 corresponding, respectively, to D. seriata isolates: CBS719.85c, R21-1a, Y46-8-1b, Y63-4-1b, Y87-3-1c, Y111-27-1,Y116-27-96, Y121-15-4, and Y125-12-1b. Lane 19: DNA molecular weight marker; the fragment size is indicated on the left.
