Development of SCAR Primers for PCR Assay to Detect Diplodia seriata
Figure 3
SCAR primers sensitivity. The amplified fragments were separated by 1.5% agarose gel electrophoresis (TBE). (a) The PCR amplifications were carried out using the primer pair DS3.8 S3-DS3.8 R4 yielding 233 bp fragment. (b) DS3.8 S3-DS3.8 R6 yielding 634 bp amplicon. Lane 1: DNA molecular weight marker; the fragment size is indicated on the right. Lanes 2 to 7 corresponding to D. seriata CBS719.85 as a template tested at decreasing concentrations: 1, 0.1, 0.01, 10−3, 10−4 , and 10−5 ng/μL, respectively.
