Table of Contents
ISRN Microbiology
Volume 2014 (2014), Article ID 836083, 7 pages
http://dx.doi.org/10.1155/2014/836083
Research Article

Investigation of Bacterial Cellulose Biosynthesis Mechanism in Gluconoacetobacter hansenii

School of Life Sciences, North Maharashtra University, P.O. Box 80, Jalgaon 425001, India

Received 28 January 2014; Accepted 25 February 2014; Published 16 March 2014

Academic Editors: J. Ruiz-Herrera and T. P. West

Copyright © 2014 Bhavna V. Mohite and Satish V. Patil. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The present study explores the mechanism of cellulose biosynthesis in Gluconoacetobacter hansenii. The cellulose synthase enzyme was purified as membrane fraction and solubilized by treatment with 0.1% digitonin. The enzyme was separated by native-gel electrophoresis and β-D-glucan analysis was carried out using in vitro gel assay. The cellulose synthase has glycoprotein nature and composed two polypeptide subunits of 93 KDa and 85 KDa. The confirmation of β-1,4-glucan (cellulose) was performed in whole and hydrolyzed monomeric sugar form. Tinopal and Congo red were used for cellulose detection on the gel. Thus the in vitro cellulose synthesis assay with cell free enzyme fraction was attempted to improve the understanding of cellulose biosynthesis.