Extractive Spectrophotometric Determination of Tenofovir Disoproxil Fumarate Using Acidic Triphenylmethane Dyes

Figure 1

Absorption spectra of tenofovir disoproxil fumarate-dye complex extracted into 10 mL chloroform: (a) drug = 20 μg mL⁻¹ + 5 mL of 0.025% BTB + 5 mL of pH 2.8 buffer; (b) drug = 25 μg mL⁻¹ + 5 mL of 0.025% BPB + 5 mL of pH 2.5 buffer; (c) drug = 25 μg mL⁻¹ + 5 mL of 0.025% BCP + 5 mL of pH 2.5 buffer; and (d) drug = 0 μg mL⁻¹ + 5 mL of 0.025% dye + 5 mL of buffer.