Extractive Spectrophotometric Determination of Tenofovir Disoproxil Fumarate Using Acidic Triphenylmethane Dyes

Table 1

Spectral and analytical parameters for the determination of TDF with triphenylmethane dyes.

Parameters	BTB	BPB	BCP
λmax (nm)	412	412	413
Beer’s law limits (µg mL^−1)	4.0–40	4.0–40	4.0–40
Molar absorptivity (L Mol^−1 cm^−1)	7462	5084	5289
Formation constant (K, M^−1)	1.342×10^6	1.256×10^6	1.42×10^6
Sandell’s sensitivity (µg cm^−2)	0.0549	0.0806	0.077
Slope (𝑎)	0.0182	0.0124	0.0129
Intercept (𝑏)	0.0499	0.0842	0.1168
Correlation coefficient (𝑟)	0.998	0.999	0.998
Limit of detection (µg mL^−1)	0.44	0.44	0.44
Limit of quantification (µg mL^−1)	1.3	1.3	1.3
Regression equation 𝑌=𝑏+𝑎𝑥	𝑌=0.05+0.0182𝑥	𝑌=0.0842+0.0124𝑥	𝑌=0.1168+0.013𝑥