Figure 1: The construct of recombinant vectors expressing colicin E2. The ColE2 gene (ceaB) and its immunity gene (ceiB) on plasmid DNA (pCoLE2-P9) were amplified using the polymerase chain reaction (PCR) with the primers. The PCR product was cleaved with BamHI and XhoI restriction endonuclease and inserted into the corresponding sites of pSLP111.3, giving rise to pSLP111.3-pCoLE2-P9.