Table of Contents
ISRN Biotechnology
Volume 2014 (2014), Article ID 909601, 7 pages
http://dx.doi.org/10.1155/2014/909601
Research Article

Use of Metagenomics and Isolation of Actinobacteria in Brazil’s Atlantic Rainforest Soil for Antimicrobial Prospecting

Laboratório de Monitoramento Ambiental/Laboratório de Biotecnologia de Microrganismos, Centro de Biotecnologia e Genética, Universidade Estadual de Santa Cruz (UESC), 45662-900 Ilhéus, BA, Brazil

Received 21 November 2013; Accepted 10 February 2014; Published 12 March 2014

Academic Editors: B. Castiglioni and M. Rossi

Copyright © 2014 Danyelle Alves Martins Assis et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. B. Spellberg, R. Guidos, D. Gilbert et al., “The epidemic of antibiotic-resistant infections: a call to action for the medical community from the infectious diseases society of America,” Clinical Infectious Diseases, vol. 46, no. 2, pp. 155–164, 2008. View at Publisher · View at Google Scholar · View at Scopus
  2. European Centre for Disease Prevention and Control/European Medicines Agency, “The bacterial challenge: time to react,” Joint Technical Report, European Centre for Disease Prevention and Control/European Medicines Agency, Stockholm, Sweden, 2009. View at Google Scholar
  3. L. Freire-Moran, B. Aronsson, C. Manz et al., “Critical shortage of new antibiotics in development against multidrug-resistant bacteria—time to react is now,” Drug Resistance Updates, vol. 14, no. 2, pp. 118–124, 2011. View at Publisher · View at Google Scholar · View at Scopus
  4. D. J. Newman and G. M. Cragg, “Natural products as sources of new drugs over the 30 years from 1981 to 2010,” Journal of Natural Products, vol. 75, no. 3, pp. 311–335, 2012. View at Publisher · View at Google Scholar · View at Scopus
  5. C. Walsh, “Antibiotics: actions, origins, resistance,” Protein Science, vol. 13, no. 11, pp. 3059–3060, 2003. View at Google Scholar
  6. M. Ventura, C. Canchaya, A. Tauch et al., “Genomics of Actinobacteria: tracing the evolutionary history of an ancient phylum,” Microbiology and Molecular Biology Reviews, vol. 71, no. 3, pp. 495–548, 2007. View at Publisher · View at Google Scholar · View at Scopus
  7. P. Kulanthaivel, A. J. Kreuzman, M. A. Strege et al., “Novel lipoglycopeptides as inhibitors of bacterial signal peptidase I,” The Journal of Biological Chemistry, vol. 279, no. 35, pp. 36250–36258, 2004. View at Publisher · View at Google Scholar · View at Scopus
  8. J. Wang, S. M. Soisson, K. Young et al., “Platensimycin is a selective FabF inhibitor with potent antibiotic properties,” Nature, vol. 441, no. 7091, pp. 358–361, 2006. View at Publisher · View at Google Scholar · View at Scopus
  9. J. Clardy and C. Walsh, “Lessons from natural molecules,” Nature, vol. 432, no. 7019, pp. 829–837, 2004. View at Publisher · View at Google Scholar · View at Scopus
  10. R. H. Baltz, “Antimicrobials from actinomycetes: back to the future,” Microbe, vol. 2, no. 3, pp. 125–131, 2007. View at Google Scholar · View at Scopus
  11. R. I. Amann, W. Ludwig, and K.-H. Schleifer, “Phylogenetic identification and in situ detection of individual microbial cells without cultivation,” Microbiological Reviews, vol. 59, no. 1, pp. 143–169, 1995. View at Google Scholar · View at Scopus
  12. J. Handelsman, M. R. Rondon, S. F. Brady, J. Clardy, and R. M. Goodman, “Molecular biological access to the chemistry of unknown soil microbes: a new frontier for natural products,” Chemistry and Biology, vol. 5, no. 10, pp. R245–R249, 1998. View at Publisher · View at Google Scholar · View at Scopus
  13. J. Handelsman, “Metagenomics: application of genomics to uncultured microorganisms,” Microbiology and Molecular Biology Reviews, vol. 68, no. 4, pp. 669–685, 2004. View at Publisher · View at Google Scholar · View at Scopus
  14. J. Bérdy, “Bioactive microbial metabolites,” The Journal of Antibiotics, vol. 58, no. 1, pp. 1–26, 2005. View at Publisher · View at Google Scholar
  15. S. A. Reis, L. V. Costa, E. D. C. Cavalcanti et al., “Protein synthesis inhibitory activity in culture filtrates from new strains of Streptomyces isolated from Brazilian tropical soils,” Letters in Applied Microbiology, vol. 37, no. 2, pp. 138–143, 2003. View at Publisher · View at Google Scholar · View at Scopus
  16. L. T. A. S. Semêdo, R. C. Gomes, A. A. Linhares et al., “Streptomyces drozdowiczii sp. nov., a novel cellulolytic streptomycete from soil in Brazil,” International Journal of Systematic and Evolutionary Microbiology, vol. 54, no. 4, pp. 1323–1328, 2004. View at Publisher · View at Google Scholar · View at Scopus
  17. J. P. da Rosa, E. Korenblum, M. N. Franco-Cirigliano et al., “Streptomyces iunalinharesii strain 235 shows the potential to inhibit bacteria involved in biocorrosion processes,” BioMed Research International, vol. 2013, Article ID 309769, 10 pages, 2013. View at Publisher · View at Google Scholar
  18. M. Tabarelli, L. P. Pinto, J. M. C. Silva, M. M. Hirota, and L. C. Bedê, “Desafios e oportunidades para a conservação da biodiversidade na Mata Atlântica brasileira,” Megadiversidade, vol. 1, no. 1, pp. 132–138, 2005. View at Google Scholar
  19. S. Omura and K. Shiomi, “Discovery, chemistry, and chemical biology of microbiol products,” Pure and Applied Chemistry, vol. 79, no. 4, pp. 581–591, 2007. View at Publisher · View at Google Scholar · View at Scopus
  20. F. E. Clark, “Aerobic spore—forming bactéria,” Methods of Soil Analysis, vol. 2, pp. 1473–1476, 1965. View at Google Scholar
  21. E. B. Shirling and D. Gottlieb, “Methods for characterization of Streptomyces species,” International Journal of Systematic Bacteriology, vol. 16, no. 3, pp. 313–340, 1966. View at Publisher · View at Google Scholar
  22. D. C. Grove and W. A. Randall, “Assay methods antibiotic activity: a laboratory manual,” Postgraduate Medical Journal, vol. 32, no. 370, article 408, 1956. View at Google Scholar
  23. W. R. Streit and R. A. Schmitz, “Metagenomics—the key to the uncultured microbes,” Current Opinion in Microbiology, vol. 7, no. 5, pp. 492–498, 2004. View at Publisher · View at Google Scholar · View at Scopus
  24. M. R. Rondon, P. R. August, A. D. Bettermann et al., “Cloning the soil metagenome: a strategy for accessing the genetic and functional diversity of uncultured microorganisms,” Applied and Environmental Microbiology, vol. 66, no. 6, pp. 2541–2547, 2000. View at Publisher · View at Google Scholar · View at Scopus
  25. J. Spížek, J. Novotná, T. Řezanka, and A. L. Demain, “Do we need new antibiotics? The search for new targets and new compounds,” Journal of Industrial Microbiology and Biotechnology, vol. 37, no. 12, pp. 1241–1248, 2010. View at Publisher · View at Google Scholar · View at Scopus
  26. T. B. A. Pessoa, S. S. de Souza, A. F. Cerqueira, and R. P. Rezende, “Construction and validation of metagenomic DNA libraries from landfarm soil microorganisms,” Genetics and Molecular Research, vol. 12, no. 2, pp. 2148–2155, 2013. View at Publisher · View at Google Scholar
  27. I. A. MacNeil, C. L. Tiong, C. Minor et al., “Expression and isolation of antimicrobial small molecules from soil DNA libraries,” Journal of Molecular Microbiology and Biotechnology, vol. 3, no. 2, pp. 301–308, 2001. View at Google Scholar · View at Scopus
  28. E. M. Gabor, W. B. L. Alkema, and D. B. Janssen, “Quantifying the accessibility of the metagenome by random expression cloning techniques,” Environmental Microbiology, vol. 6, no. 9, pp. 879–886, 2004. View at Publisher · View at Google Scholar · View at Scopus
  29. S. Donadio, P. Monciardini, and M. Sosio, “Polyketide synthases and nonribosomal peptide synthetases: the emerging view from bacterial genomics,” Natural Product Reports, vol. 24, no. 5, pp. 1073–1109, 2007. View at Publisher · View at Google Scholar · View at Scopus
  30. L. T. A. S. Semêdo, A. A. Linhares, R. C. Gomes et al., “Isolation and characterization of actinomycetes from Brazilian tropical soils,” Microbiological Research, vol. 155, no. 4, pp. 291–299, 2001. View at Publisher · View at Google Scholar · View at Scopus
  31. R. F. de Souza, R. R. R. Coelho, A. Macrae et al., “Streptomyces lunalinharesii sp. nov., a chitinolytic streptomycete isolated from cerrado soil in Brazil,” International Journal of Systematic and Evolutionary Microbiology, vol. 58, no. 12, pp. 2774–2778, 2008. View at Publisher · View at Google Scholar · View at Scopus
  32. D. R. Sacramento, R. R. R. Coelho, M. D. Wigg et al., “Antimicrobial and antiviral activities of an actinomycete (Streptomyces sp.) isolated from a Brazilian tropical forest soil,” World Journal of Microbiology and Biotechnology, vol. 20, no. 3, pp. 225–229, 2004. View at Publisher · View at Google Scholar · View at Scopus
  33. V. P. Gullo, J. McAlpine, K. S. Lam, D. Baker, and F. Petersen, “Drug discovery from natural products,” Journal of Industrial Microbiology and Biotechnology, vol. 33, no. 7, pp. 523–531, 2006. View at Publisher · View at Google Scholar · View at Scopus
  34. M. A. Fischbach and C. T. Walsh, “Antibiotics for emerging pathogens,” Science, vol. 325, no. 5944, pp. 1089–1093, 2009. View at Publisher · View at Google Scholar · View at Scopus
  35. M. C. Enright, D. A. Robinson, G. Randle, E. J. Feil, H. Grundmann, and B. G. Spratt, “The evolutionary history of methicillin-resistant Staphylococcus aureus (MRSA),” Proceedings of the National Academy of Sciences of the United States of America, vol. 99, no. 11, pp. 7687–7692, 2002. View at Publisher · View at Google Scholar · View at Scopus
  36. W. H. Traub and B. Leonhard, “Heat stability of the antimicrobial activity of sixty-two antibacterial agents,” Journal of Antimicrobial Chemotherapy, vol. 35, no. 1, pp. 149–154, 1995. View at Publisher · View at Google Scholar · View at Scopus
  37. M. Hassani, R. Lázaro, C. Pérez, S. Condón, and R. Pagán, “Thermostability of oxytetracycline, tetracycline, and doxycycline at ultrahigh temperatures,” Journal of Agricultural and Food Chemistry, vol. 56, no. 8, pp. 2676–2680, 2008. View at Publisher · View at Google Scholar · View at Scopus
  38. J. Samelis, G. K. Bedie, J. N. Sofos, K. E. Belk, J. A. Scanga, and G. C. Smith, “Control of Listeria monocytogenes with combined antimicrobials after postprocess contamination and extended storage of frankfurters at 4°C in vacuum packages,” Journal of Food Protection, vol. 65, no. 2, pp. 299–307, 2002. View at Google Scholar · View at Scopus