Table of Contents
International Scholarly Research Notices
Volume 2014, Article ID 935689, 7 pages
http://dx.doi.org/10.1155/2014/935689
Research Article

Hydroxyapatite-Based Colloidal Gels Facilitate the Proliferation and Migration of Chondrocytes and the Adhesion of Umbilical Cord Mesenchymal Stem Cells

1Division of Molecular Biosciences, University of Kansas, Haworth Hall, 1200 Sunnyside Avenue, Lawrence, KS 66045, USA
2Rockhurst University, Kansas City, MO 64109, USA
3Ascend Technology, Dallas, TX 75034, USA
4School of Engineering, University of Kansas, Lawrence, KS 66045, USA

Received 22 July 2014; Revised 26 October 2014; Accepted 27 October 2014; Published 29 December 2014

Academic Editor: Madaswamy S. Muthu

Copyright © 2014 Syed A. Jamal and Qiang Ye. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Collective movement of cells that have been delivered on biomaterials for transplantation purposes would be a desirable attribute that would promote wound healing, cell proliferation, and eventual growth and regeneration of damaged organs. We hypothesized that colloidal gels made from hydroxyapatite (HA) and poly(D,L-lactic-co-glycolic acid) (PLGA) particles will be conducive to the growth and migration of porcine chondrocytes, will allow the adhesion of human umbilical cord mesenchymal stem cells, and will have negligible effects on the cell cycle of these cells. Then, we performed experiments designed to assess the viability and migratory properties of porcine chondrocytes studded on nanosized HA/PLGA particles. Our experiments show that porcine chondrocytes migrated in and around a hydroxyapatite-based biomaterial that could be described as a colloidal gel. Cells in the colloidal gel demonstrated unidirectional movement. Cells were seen to be extending lamellae and were followed by other cells.