Entrapment of -Amylase in Agar Beads for Biocatalysis of Macromolecular Substrate

Table 1

Effect of different concentrations of agar, calcium chloride, and hardening time on the percentage entrapment of α-amylase and particle size of bead formed.

Agar concentration	Calcium chloride concentration	Hardening time (min)	% entrapment	Particle size (mm)
2%	0 mM	20 min	49.78	0.482 ± 2.92
		30 min	45.35	0.477 ± 1.18
	50 mM	10 min	63.87	0.497 ± 2.21
		20 min	61.32	0.497 ± 1.17
		30 min	58.96	0.485 ± 2.20
	75 mM	10 min	62.50	0.466 ± 1.19
		20 min	57.59	0.461 ± 1.22
		30 min	56.21	0.459 ± 2.18
	100 mM	10 min	55.22	0.460 ± 3.17
		20 min	54.25	0.462 ± 2.15
		30 min	52.86	0.462 ± 2.22
3%	0 mM	20 min	63.83	0.844 ± 3.19
		30 min	61.99	0.842 ± 2.17
	50 mM	10 min	82.68	0.715 ± 2.21
		20 min	78.00	0.708 ± 1.17
		30 min	74.40	0.701 ± 1.20
	75 mM	10 min	82.81	0.682 ± 2.19
		20 min	80.00	0.677 ± 3.22
		30 min	76.26	0.671 ± 3.18
	100 mM	10 min	76.20	0.672 ± 2.21
		20 min	74.68	0.669 ± 1.23
		30 min	73.11	0.663 ± 2.18
4%	0 mM	20 min	64.05	1.12 ± 3.21
		30 min	62.03	1.11 ± 2.19
	50 mM	10 min	78.81	1.02 ± 3.17
		20 min	76.45	1.01 ± 3.20
		30 min	74.88	1.01 ± 2.19
	75 mM	10 min	76.06	0.96 ± 3.22
		20 min	73.51	0.95 ± 2.20
		30 min	71.54	0.95 ± 3.21
	100 mM	10 min	73.11	0.92 ± 3.16
		20 min	70.75	0.91 ± 3.18
		30 min	69.18	0.91 ± 3.21