Entrapment of -Amylase in Agar Beads for Biocatalysis of Macromolecular Substrate

Table 2

Thermodynamic parameters of free and entrapped α-amylase.

Temperature (Kelvin)	Free enzyme			Agar entrapped enzyme			Calcium agar entrapped enzyme
	(s^−1)	(kcal/mol)	Half-life (min)	(s^−1)	(kcal/mol)	Half-life (min)	(s^−1)	(kcal/mol)	Half-life (min)
323	1.71	17.60	67.77	1.52	20.04	76.11	1.03	27.49	111.58
328	2.83	17.60	40.75	2.27	20.04	50.91	1.32	27.49	87.64
333	4.47	17.60	25.85	3.29	20.04	35.06	2.02	27.49	57.07
338	6.40	17.60	18.05	5.02	20.04	23.03	3.65	27.49	31.62
343	—	—	—	—	—	—	6.97	27.49	16.59