Table of Contents
ISRN Computational Biology
Volume 2014, Article ID 960902, 7 pages
Research Article

In Silico Proteome Cleavage Reveals Iterative Digestion Strategy for High Sequence Coverage

Department of Chemistry and Biochemistry, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0378, USA

Received 4 February 2014; Accepted 17 March 2014; Published 22 April 2014

Academic Editors: Y. Cai and J. Ruan

Copyright © 2014 Jesse G. Meyer. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplemental figure legend 1: Effect of missed cleavages on peptide length distributions produced for in silico digestion with AspN. Ten simulations each of 1%, 10%, and 50% missed cleavage propensity were run and each distribution was plotted. As the missed cleavage propensity increases, the peptides become larger and the variance increases.

  1. Supplementary Figure