The Mre11 Cellular Protein Is Modified by Conjugation of Both SUMO-1 and SUMO-2/3 during Adenovirus Infection
Figure 2
The Mre11 protein colocalizes with E4 Orf3 and E1B 55 kDa in nuclear and cytoplasmic structures throughout the Ad5 replication cycle. The intracellular distribution of (a) E4 Orf3 (6A11), Mre11 (12D7-GeneTex), and vimentin and (b) E4 Orf3 (6A11), E1B 55 kDa (2A6), and vimentin was analyzed in mock-infected and Ad5-infected HFF cells at various times after infection. The cells were processed for immunofluorescence and E4 Orf3, Mre11, E1B 55 kDa, and vimentin were visualized as described in Section 2. The E4 Orf3 protein signal is shown in yellow, Mre11 (a) or E1B 55 kDa (b) in red, vimentin in green, and DAPI-stained nuclei in blue.
