The Mre11 Cellular Protein Is Modified by Conjugation of Both SUMO-1 and SUMO-2/3 during Adenovirus Infection
Figure 5
Ad5-infection induces the nuclear reorganization of SUMO-1 and SUMO-2/3 paralogues. (a) The intracellular distribution of E4 Orf3, Mre11, and SUMO-1 was analyzed in HFF cells infected with Ad5, the A143, or the E1B 55 kDa, E4 Orf6, or E4 Orf3 null mutants at 28 h p.i. After infection cells were processed for immunofluorescence as described in Section 2. E4 Orf3 is shown in magenta, Mre11 in red, and SUMO-1 in green. (b) The intracellular distribution of E4 Orf3 and SUMO-2/3 was analyzed in HFF cells infected with Ad5, A143, or the null mutants for E1B 55 kDa, E4 Orf6, or E4 Orf3. E4 Orf3 is shown in red and SUMO-2/3 in green; DAPI-stained nuclei are shown in blue. Insets were enlarged 5X to show the localization of SUMO-2/3 in ring-like structures, adjacent to E4 Orf3 tracks. (c) The intracellular distribution of DBP and SUMO-2/3 was analyzed in HFF cells infected with the Ad5, A143, or the null mutants for E1B 55 kDa or E4 Orf3 viruses, at 28 h p.i. DBP is shown in red and SUMO-2/3 in green; DAPI-stained nuclei are shown in blue.
