Table of Contents
International Scholarly Research Notices
Volume 2015 (2015), Article ID 464376, 7 pages
http://dx.doi.org/10.1155/2015/464376
Research Article

Isolation of Infectious Bursal Disease Virus Using Indigenous Chicken Embryos in Kenya

1State Department of Livestock, Regional Veterinary Investigation Laboratories, P.O. Box 204-80113, Mariakani, Kenya
2University of Nairobi, P.O. Box 29053-00625, Nairobi, Kenya
3State Department of Livestock, Central Veterinary Laboratories, Private Bag Box 00625, Nairobi, Kenya
4Kenya Veterinary Vaccine Production Institute (KEVEVAPI), P.O. Box 53260-00200, Nairobi, Kenya

Received 29 July 2015; Revised 22 October 2015; Accepted 1 November 2015

Academic Editor: Tom Aire

Copyright © 2015 W. U. Mutinda et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Infectious bursal disease virus (IBDV) isolates were recovered from outbreaks to initiate activities towards developing a local vaccine strain. Use of indigenous chicken embryos was exploited to determine their potential, promote utilization of local resources for research, and enhance household economic activities. Bursa of Fabricius (BFs) samples from outbreaks shown to be IBDV positive was homogenized and inoculated in 4-week-old specific pathogen-free (SPF) IBDV seronegative white leghorn chicks. The harvested virus was inoculated into 11-day-old indigenous chicken embryos that were IBDV seronegative and passaged serially three times after which they were inoculated into 4-week-old indigenous chicks to test for presence and virulence of propagated virus. Out of 153 BFs collected from outbreaks, 43.8% (67/153) were positive for IBDV antigen and 65.7% (44/67) caused disease in SPF chicks. The embryo mean mortalities were 88% on primary inoculation, 94% in 1st passage, 91% in 2nd passage, and 67% in 3rd passage. After the third passage in embryos all the 44 isolates were virulent in 4-week-old indigenous chicks. The results show that indigenous chicken embryos support growth of IBDV and can be used to propagate the virus as an alternative viral propagating tool for respective vaccine preparation.