Table of Contents
International Scholarly Research Notices
Volume 2015, Article ID 489547, 6 pages
http://dx.doi.org/10.1155/2015/489547
Research Article

Effect of In Vitro Testing Parameters on Ceftazidime-Avibactam Minimum Inhibitory Concentrations

Cerexa, Inc., Oakland, CA 94612, USA

Received 13 February 2015; Accepted 16 April 2015

Academic Editor: Wladimir Sougakoff

Copyright © 2015 Tiffany R. Keepers et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Effects of varying in vitro susceptibility testing parameters of the broth microdilution assay on ceftazidime-avibactam MICs were determined and compared to meropenem and piperacillin-tazobactam for 9 Enterobacteriaceae and 4 Pseudomonas aeruginosa isolates. The effect of varying incubation conditions (ambient air or 5% CO2), pH of medium, medium composition (cation-adjusted Mueller Hinton Broth with and without laked horse blood and Haemophilus Test Medium), cation content of the medium, and inoculum density were tested. Most variations had no effect on ceftazidime-avibactam MIC values (no more than a 2-fold change). However, acidic pH or high inoculum resulted in 4- to 16-fold changes in MIC, which was similar to those observed for meropenem and piperacillin-tazobactam under these conditions. Overall, this study shows that slight variations in testing parameters during routine MIC testing will likely have no significant effect on ceftazidime-avibactam MIC values.