Table of Contents
International Scholarly Research Notices
Volume 2017, Article ID 8934285, 9 pages
https://doi.org/10.1155/2017/8934285
Research Article

Diversity of Aerobic Bacteria Isolated from Oral and Cloacal Cavities from Free-Living Snakes Species in Costa Rica Rainforest

1Laboratorio de Análisis Genómico, Escuela de Ciencias Biológicas, Universidad Nacional, 86-3000 Heredia, Costa Rica
2Laboratorio de Biología Molecular, Universidad de Costa Rica, Sede del Atlántico, Turrialba, Costa Rica
3Centro de Investigación en Enfermedades Tropicales, Facultad de Microbiología, Universidad de Costa Rica, San José, Costa Rica

Correspondence should be addressed to Rodolfo Umaña-Castro; rc.anu@ortsac.anamu.oflodor

Received 5 April 2017; Revised 28 June 2017; Accepted 6 July 2017; Published 20 August 2017

Academic Editor: Giovanni Colonna

Copyright © 2017 Allan Artavia-León et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Costa Rica has a significant number of snakebites per year and bacterial infections are often complications in these animal bites. Hereby, this study aims to identify, characterize, and report the diversity of the bacterial community in the oral and cloacal cavities of venomous and nonvenomous snakes found in wildlife in Costa Rica. The snakes where captured by casual encounter search between August and November of 2014 in the Quebrada González sector, in Braulio Carrillo National Park. A total of 120 swabs, oral and cloacal, were taken from 16 individuals of the Viperidae and Colubridae families. Samples were cultured on four different media at room temperature. Once isolated in pure culture, colonies were identified with the VITEK® 2C platform (bioMérieux). In order to test the identification provided on environmental isolates, molecular analyses were conducted on 27 isolates of different bacterial species. Specific 16S rDNA PCR-mediated amplification for bacterial taxonomy was performed, then sequenced, and compared with sequences of Ribosomal Database Project (RDP). From 90 bacterial isolates, 40 different bacterial species were identified from both oral and cloacal swabs. These results indicate the diversity of opportunistic pathogens present and their potential to generate infections and zoonosis in humans.