Effects of β𝟐 Agonists, Corticosteroids, and Novel Therapies on Rhinovirus-Induced Cytokine Release and Rhinovirus Replication in Primary Airway Fibroblasts

Figure 1

(a) Time course of RV replication. Concentration is of RV from infected fibroblasts (MOI=0.1) at 0, 3, 6, 24, 48 and 72 hours post infection were measured by RV titration. RV concentration was compared with each time point post infection using a 1-way ANOVA (𝑛=5). (b,c) Time course of RV-induced IL-6 and IL-8. Concentration of (b) IL-6 and (c) IL-8 release from noninfected fibroblast (constitutive release) or UVi-RV-(UVi-) or RV-16-(RV-) infected fibroblasts (MOI=0.1) at 0, 3, 6, 24, 48 and 72 hours post infection were measured by ELISA. RV-induced IL-6 and IL-8 at 48, and 72 hours post infection compared to control and UVi (2-way ANOVA, 𝑛=5). All data are presented as mean ± SEM. Significance of comparisons is represented as *𝑃<0.05, **𝑃<0.01, and ***𝑃<0.0001.

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