Interleukin-4 in the Generation of the AERD Phenotype: Implications for Molecular Mechanisms Driving Therapeutic Benefit of Aspirin Desensitization
COX-2 protein expression in resting and LPS-stimulated monocytes. Monocytes were isolated from blood by magnetic bead purification. Cells were treated with IL-4 (10 ng/mL), IL-13 (10 ng/mL), or LPS (1 μg/mL) for 24 hrs and whole cell lysates collected. Proteins were separated on a 10% SDS acrylamide gel and transferred to nitrocellulose. The membrane was probed with anti-COX-2 and then stripped and reprobed with anti-β-actin.
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