Interleukin-4 in the Generation of the AERD Phenotype: Implications for Molecular Mechanisms Driving Therapeutic Benefit of Aspirin Desensitization

Figure 5

Western hybridization of nuclear extracts. Nuclear extracts obtained as described for Figure 4 were electrophoresed on a 10% SDS polyacrylamide gel and transferred to a nitrocellulose membrane. Presence of phosphoSTAT6 was determined via probing with anti-phosphoSTAT6 antibodies and a secondary peroxidase-labeled antibody [43].