Interleukin-4 in the Generation of the AERD Phenotype: Implications for Molecular Mechanisms Driving Therapeutic Benefit of Aspirin Desensitization

Table 2

PgE₂ metabolic pathway gene expression in control and AERD tissue.

Gene	Control ΔCT^1	AERD ΔCT	ΔΔCT (2^ΔΔCT)^2
COX-1	10.5±0.4^1	11.3±2.1	−0.8 (.57)
COX-2	6.0±0.6	7.9±2.8 *	−1.9 (.27)
mPGES-1	4.0±0.7	5.0±0.5	−1 (.5)
mPGES-2	2.8±0.8	4.5±0.4 *	−1.7 (.31)
cPGES	−0.1±0.6	0.0±0.5	+0.1 (1.07)

* 𝑃<0.05.
¹Quantitiatve polymerase chain reaction data are presented as ΔC_T, which is the difference in threshold cycle of expression of each gene compared to housekeeping gene (each cycle corresponds to ~1 log₂ difference in mRNA concentration; a higher ΔC_T represents less mRNA).
²ΔΔC_T is the difference in ΔC_T of gene expressed in control compared to AERD tissue.
2^ΔΔCT is the relative expression of gene in AERD compared to control tissue.