Cyclin D1 in ASM Cells from Asthmatics Is Insensitive to Corticosteroid Inhibition
Figure 4
Dexamethasone-induced GR nuclear translocation does not differ between mitogen-treated ASM from asthmatics as compared to non-asthmatics. ASM cells from (a) non-asthmatics or (b) asthmatics were pretreated for 1 h with vehicle or dexamethasone (100 nM) and then stimulated for 1 h with 25 ng/mL PDGF-BB. Cytoplasmic (CYT) and nuclear (NUC) fractions were prepared, and GR protein was measured by western blotting (representative blots illustrated), using α-tubulin and lamin A/C as the loading controls for the cytoplasmic or nuclear fractions, respectively.