Calreticulin Is a Negative Regulator of Bronchial Smooth Muscle Cell Proliferation
Figure 3
(a) Immunoanalyses demonstrating the effect of asthma drugs on the C/EBPα (p42), C/EBPα (p30), and calreticulin expression pattern. BSM cells were untreated or incubated with 5% FBS alone, and in the presence of budesonide (10−8 M) or formoterol (10−8 M) for 24 and 96 hours. (b) Immunoanalyses demonstrating C/EBPα (p42), C/EBPα (p30), and calreticulin expression patterns in BSM cells after transient knockout of the calreticulin by siRNA. BSM cells were untreated or incubated with 5% FBS alone, and in the presence of formoterol (10−8 M) for 24 hours. (c) BSM cell proliferation (presented as relative cell counts) in response to PDGF-BB (5 ng/mL) and the effect of increasing concentrations calreticulin-specific siRNA relative to control siRNA (as indicated). *Significant inhibition relative to PDGF-BB-stimulated cells (; ).
