Table of Contents
Journal of Allergy
Volume 2012, Article ID 862023, 7 pages
Research Article

Evaluation of a Novel Rapid Test System for the Detection of Specific IgE to Hymenoptera Venoms

1Allergy Research Group, Department of Dermatology, University Medical Center Freiburg, Hauptstra├če 7, 79104 Freiburg, Germany
2Dr. Fooke Laboratorien GmbH, Habichtweg 16, 41468 Neuss, Germany

Received 15 September 2011; Revised 25 November 2011; Accepted 26 November 2011

Academic Editor: Ting Fan Leung

Copyright © 2012 Nikolai Pfender et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Background. The Allergy Lateral Flow Assay (ALFA) is a novel rapid assay for the detection of sIgE to allergens. The objective of this study is the evaluation of ALFA for the detection of sIgE to bee venom (BV) and wasp venom (WV) in insect venom allergic patients. Methods. Specific IgE to BV and WV was analyzed by ALFA, ALLERG-O-LIQ, and ImmunoCAP in 80 insect venom allergic patients and 60 control sera. Sensitivity and specificity of ALFA and correlation of ALFA and ImmunoCAP results were calculated. Results. The sensitivity/specificity of ALFA to the diagnosis was 100%/83% for BV and 82%/97% for WV. For insect venom allergic patients, the Spearman correlation coefficient for ALFA versus ImmunoCAP was 0.79 for BV and 0.80 for WV. However, significant differences in the negative control groups were observed. Conclusion. ALFA represents a simple, robust, and reliable tool for the rapid detection of sIgE to insect venoms.