Evaluation of Differentiated Human Bronchial Epithelial Cell Culture Systems for Asthma Research

Table 1

Antibodies used for immunofluorescent staining of cultured cells.

Target	Antibody	Dilution	Secondary
β-Tubulin IV	Sigma T7941	1 : 500	Goat anti-Mouse FITC; (Sigma F0257) 1 : 100
MUC5AC	Abcam AB3649	1 : 250
E-Cadherin	Millipore MAB3199Z	1 : 500
Mouse IgG	Isotype Sigma M7894	1 : 463
ZO-1	Invitrogen 40-2200	1 : 125	Goat anti-Rabbit Rhodamine; TRITC (Stratech 111-025-003) 1 : 100
Rabbit IgG	Isotype Abcam AB27472	1 : 1