Journal of Allergy / 2012 / Article / Tab 3

Research Article

Evaluation of Differentiated Human Bronchial Epithelial Cell Culture Systems for Asthma Research

Table 3

A summary of outcomes for different cell types. For β-Tubulin IV and MUC5AC, “localised” expression refers to expression in a subset of cells. For E-Cadherin and ZO-1, expression was “localised” to the cell boundaries. *staining was similar to isotype control. HBEC D1 is Donor 1 and HBEC D2 is Donor 2.

HBEC D1HBEC D2Calu-3BEAS-2BBEAS-2B R1

TEERvariablevariablehighlownone

β-Tubulin IVmRNAlowlowhighmidhigh
proteinmidmidmidhigh mid
localisedlocaliseddiffuselocalisedlocalised

MUC5ACmRNAhighhighhighnonenone
proteinhighhighhighlownone
localisedlocalisedlocaliseddiffuse

E-CadherinmRNAhighhighmidmidnone
proteinhighhighhighmidlow
localisedlocalisedlocalisedpart localiseddiffuse

ZO-1mRNAhighhighlowmidmid
proteinhighhighlowmid*low*
localisedlocalisedlocalisedpart localiseddiffuse

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