Table of Contents
Journal of Allergy
Volume 2012 (2012), Article ID 958383, 10 pages
Research Article

Reticular Basement Membrane Vessels Are Increased in COPD Bronchial Mucosa by Both Factor VIII and Collagen IV Immunostaining and Are Hyperpermeable

Menzies Research Institute, University of Tasmania, Private Bag 23, Hobart, TAS 7000, Australia

Received 15 August 2011; Accepted 28 November 2011

Academic Editor: Georgia Hardavella

Copyright © 2012 Amir Soltani et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Background and Objective. Using Collagen IV staining, we have previously reported that the reticular basement membrane (Rbm) is hypervascular and the lamina propria (LP) is hypovascular in COPD airways. This study compared Collagen IV staining with vessels marked with anti-Factor VIII and examined vessel permeability in bronchial biopsies from COPD and normal subjects using albumin staining. Results. Anti-Collagen IV antibody detected more vessels in the Rbm (P=0.002) and larger vessels in both Rbm (P<0.001) and LP (P=0.003) compared to Factor VIII. COPD airways had more vessels (with greater permeability) in the Rbm (P=0.01) and fewer vessels (with normal permeability) in the LP compared to controls with both Collagen IV and Factor VIII antibodies (P=0.04 and P=0.01). Conclusion. Rbm vessels were increased in number and were hyperpermeable in COPD airways. Anti-Collagen IV and anti-Factor VIII antibodies did not uniformly detect the same vessel populations; the first is likely to reflect larger and older vessels with the latter reflecting smaller, younger vessels.