Impact of Adiponectin Overexpression on Allergic Airways Responses in Mice

Figure 5

Histological sections of airways of wildtype (WT) and adiponectin transgenic (Adipo Tg) mice sensitized with ovalbumin (OVA) without alum and challenged intranasally with OVA or PBS every week for 4 weeks (chronic). Light micrographs showing H&E (panels (a)–(d)) or PAS (panels (e)–(h)) staining. Arrowheads in (a)–(d) indicate regions of inflammatory cell accumulation. Arrows in (e)–(h) indicate regions of “purple” staining that were recognized by Image Pro software (Olympus) during quantification of goblet cell abundance (see text). The bars in panels (a) and (e) = 10 μm and correspond to all images in the upper and lower row, respectively. PAS staining for mucous-containing goblet cells ((e)–(h)) is from medium sized peripheral airways.