Immunochemical Characterization of Acacia Pollen Allergens and Evaluation of Cross-Reactivity Pattern with the Common Allergenic Pollens

Figure 1

(a) Coomassie Brilliant Blue stained SDS-PAGE of the crude extract of A. farnesiana pollen in reducing and nonreducing conditions on 12.5% acrylamide gel. Lane MW, molecular weight marker (Fermentas, Lithuania); R, reducing condition; NR, nonreducing condition. (b) Immunoblotting of Acacia pollen extract (with reducing SDS-PAGE). Each strip was first blotted with Acacia pollen extract. All strips were then incubated with allergic patients’ sera and detected for IgE reactive protein bands. Lane MW, low molecular weight. NC, negative control.