Immunochemical Characterization of Acacia Pollen Allergens and Evaluation of Cross-Reactivity Pattern with the Common Allergenic Pollens

Figure 3

Immunoblotting inhibition assays. Lane MW, molecular weight marker (Fermentas, Lithuania). Lane 1, Acacia protein strip incubated with pooled serum without inhibitor (negative control). Lane 2, Acacia protein strip incubated with pooled serum containing 70 μg of Acacia pollen extract as inhibitor (positive control). Lane 3, Acacia protein strip incubated with pooled serum containing 70 μg P. juliflora as inhibitor. Lane 4, Acacia protein strip incubated with pooled serum containing 70 μg S. kali as inhibitor. Lane 5, Acacia protein strip incubated with pooled serum containing 70 μg A. retroflexus as inhibitor. Lane 6, Acacia protein strip incubated with pooled serum containing 70 μg C. album as inhibitor. Lane 7, Acacia protein strip incubated with pooled serum containing 70 μg K. scoparia as inhibitor.