Human Lung Mast Cell Products Regulate Airway Smooth Muscle CXCL10 Levels

Figure 4

The proteolytic effects of tryptase on rhCXCL10 in a cell free system. The rhCXCL10 made up in serum-free DMEM supplemented with 0.1% BSA was placed in 96-well culture plates and incubated with tryptase ± leupeptin, heat-inactivated tryptase (HI), or its vehicle for 24 h under the same conditions as the ASM cells. CXCL10 was detected using ELISA. ^∗𝑃<0.05 compared with the vehicle control, ANOVA.