Cloning and Expression of Ama r 1, as a Novel Allergen of Amaranthus retroflexus Pollen

Figure 5

Immunoblotting inhibition assays. Lane MW: molecular weight marker (GE Healthcare, UK); lane 1: A. retroflexus protein strip incubated with pooled sera without inhibitor (negative control); lane 2: A. retroflexus protein strip incubated with pooled sera containing 65 μg of A. retroflexus pollen extract as an inhibitor (positive control); and lane 3: A. retroflexus protein strip incubated with pooled sera containing 10 μg purified rAma r 1, as an inhibitor.