Table of Contents
Journal of Allergy
Volume 2016 (2016), Article ID 4092817, 8 pages
http://dx.doi.org/10.1155/2016/4092817
Research Article

Cloning and Expression of Ama r 1, as a Novel Allergen of Amaranthus retroflexus Pollen

1Department of Immunology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz 1478736714, Iran
2Department of Immunology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran

Received 6 August 2015; Accepted 28 December 2015

Academic Editor: Desiderio Passali

Copyright © 2016 Payam Morakabati et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. M.-A. Assarehzadegan, A. Shakurnia, and A. Amini, “The most common aeroallergens in a tropical region in Southwestern Iran,” World Allergy Organization Journal, vol. 6, no. 1, article 7, 2013. View at Publisher · View at Google Scholar · View at Scopus
  2. D. Ghosh, P. Chakraborty, J. Gupta et al., “Associations between pollen counts, pollutants, and asthma-related hospital admissions in a high-density indian metropolis,” Journal of Asthma, vol. 49, no. 8, pp. 792–799, 2012. View at Publisher · View at Google Scholar · View at Scopus
  3. K. Min, M. Yoshida, R. Miike, and E. Tam, “Aeroallergen sensitivity in Hawaii: association with asthma and increased prevalence of sensitivity to indoor allergens Since 1996,” Hawaii Journal of Medicine & Public Health, vol. 73, no. 9, supplement 1, pp. 9–12, 2014. View at Google Scholar
  4. M. Villalba, R. Barderas, S. Mas, C. Colás, E. Batanero, and R. Rodríguez, “Amaranthaceae pollens: review of an emerging allergy in the mediterranean area,” Journal of Investigational Allergology and Clinical Immunology, vol. 24, no. 6, pp. 371–381, 2014. View at Google Scholar · View at Scopus
  5. M.-A. Assarehzadegan, A. H. Shakurnia, and A. Amini, “Sensitization to common aeroallergens among asthmatic patients in a tropical region affected by dust storm,” Journal of Medical Sciences, vol. 13, no. 7, pp. 592–597, 2013. View at Publisher · View at Google Scholar · View at Scopus
  6. M. Tehrani, M. Sankian, M. A. Assarehzadegan, R. Falak, F. Jabbari, and A. Varasteh, “Immunochemical characterization of Amaranthus retroflexus pollen extract: extensive cross-reactive allergenic components among the four species of Amaranthaceae/Chenopodiaceae,” Iranian Journal of Allergy, Asthma and Immunology, vol. 9, no. 2, pp. 87–95, 2010. View at Google Scholar · View at Scopus
  7. P. A. Wurtzen, H. S. Nelson, H. Lowenstein, and H. Ipsen, “Characterization of chenopodiales (Amaranthus retroflexus, Chenopodium album, Kochia scoparia, Salsola pestifer) pollen allergens,” Allergy, vol. 50, no. 6, pp. 489–497, 1995. View at Publisher · View at Google Scholar · View at Scopus
  8. M. Tehrani, M. Sankian, M. A. Assarehzadegan et al., “Identification of a new allergen from Amaranthus retroflexus pollen, Ama r 2,” Allergology International, vol. 60, no. 3, pp. 309–316, 2011. View at Publisher · View at Google Scholar · View at Scopus
  9. S. M. Hasnain, A. R. Al-Frayh, J. L. Subiza et al., “Sensitization to indigenous pollen and molds and other outdoor and indoor allergens in allergic patients from Saudi Arabia, United Arab Emirates, and Sudan,” World Allergy Organization Journal, vol. 5, no. 6, pp. 59–65, 2012. View at Publisher · View at Google Scholar · View at Scopus
  10. A. B. Singh and P. Dahiya, “Antigenic and allergenic properties of Amaranthus spinosus pollen—a commonly growing weed in India,” Annals of Agriculture and Environmental Medicine, vol. 9, no. 2, pp. 147–151, 2002. View at Google Scholar · View at Scopus
  11. J. de Dios Alché, M. M'rani-Alaoui, A. J. Castro, and M. I. Rodríguez-García, “Ole e 1, the major allergen from olive (Olea europaea L.) pollen, increases its expression and is released to the culture medium during in vitro germination,” Plant and Cell Physiology, vol. 45, no. 9, pp. 1149–1157, 2004. View at Publisher · View at Google Scholar · View at Scopus
  12. R. Barderas, M. Villalba, M. Lombardero, and R. Rodríguez, “Identification and characterization of Che a 1 allergen from Chenopodium album pollen,” International Archives of Allergy and Immunology, vol. 127, no. 1, pp. 47–54, 2002. View at Publisher · View at Google Scholar · View at Scopus
  13. L. Castro, S. Mas, R. Barderas et al., “Sal k 5, a member of the widespread ole e 1-like protein family, is a new allergen of russian thistle (Salsola kali) pollen,” International Archives of Allergy and Immunology, vol. 163, no. 2, pp. 142–153, 2014. View at Publisher · View at Google Scholar · View at Scopus
  14. R. Barderas, A. Purohit, R. Rodríguez, G. Pauli, and M. Villalba, “Isolation of the main allergen Fra e 1 from ash (Fraxinus excelsior) pollen: comparison of the natural and recombinant forms,” Annals of Allergy, Asthma & Immunology, vol. 96, no. 4, pp. 557–563, 2006. View at Publisher · View at Google Scholar · View at Scopus
  15. E. Batanero, M. A. Gonzalez De La Peña, M. Villalba, R. I. Monsalve, M. Martin-Esteban, and R. Rodriguez, “Isolation, cDNA cloning and expression of Lig v 1, the major allergen from privet pollen,” Clinical and Experimental Allergy, vol. 26, no. 12, pp. 1401–1410, 1996. View at Publisher · View at Google Scholar · View at Scopus
  16. E. González, M. Villalba, and R. Rodríguez, “Immunological and molecular characterization of the major allergens from lilac and privet pollens overproduced in Pichia pastoris,” Clinical and Experimental Allergy, vol. 31, no. 2, pp. 313–321, 2001. View at Publisher · View at Google Scholar · View at Scopus
  17. M. H. Shamsbiranvand, A. Khodadadi, M.-A. Assarehzadegan, S. H. Borci, and A. Amini, “Immunochemical characterization of acacia pollen allergens and evaluation of cross-reactivity pattern with the common allergenic pollens,” Journal of Allergy, vol. 2014, Article ID 409056, 7 pages, 2014. View at Publisher · View at Google Scholar
  18. F. Zarinhadideh, A. Amini, M. A. Assarehzadegan, S. H. Borsi, N. Sepahi, and H. Ali-Sadeghi, “Immunochemical and molecular characterization of allergenic profilin (Koc s 2) from Kochia scoparia pollen,” Journal of the Korean Society for Applied Biological Chemistry, vol. 58, no. 3, pp. 443–451, 2015. View at Publisher · View at Google Scholar
  19. M. M. Bradford, “A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding,” Analytical Biochemistry, vol. 72, no. 1-2, pp. 248–254, 1976. View at Publisher · View at Google Scholar · View at Scopus
  20. P. Chomczynski and N. Sacchi, “Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction,” Analytical Biochemistry, vol. 162, no. 1, pp. 156–159, 1987. View at Publisher · View at Google Scholar · View at Scopus
  21. J. A. Asturias, M. C. Arilla, N. Gómez-Bayón, J. Martínez, A. Martínez, and R. Palacios, “Cloning and expression of the panallergen profilin and the major allergen (Ole e 1) from olive tree pollen,” Journal of Allergy and Clinical Immunology, vol. 100, no. 3, pp. 365–372, 1997. View at Publisher · View at Google Scholar · View at Scopus
  22. B. N. Calabozo, A. Díaz-Perales, G. Salcedo, D. Barber, and F. Polo, “Cloning and expression of biologically active Plantago lanceolata pollen allergen Pla l 1 in the yeast Pichia pastoris,” Biochemical Journal, vol. 372, no. 3, pp. 889–896, 2003. View at Publisher · View at Google Scholar · View at Scopus
  23. A.-R. Varasteh, M. Moghadam, F. Vahedi, T. Kermani, and M. Sankian, “Cloning and expression of the allergen Cro s 2 profilin from saffron (Crocus sativus),” Allergology International, vol. 58, no. 3, pp. 429–435, 2009. View at Publisher · View at Google Scholar · View at Scopus
  24. U. K. Laemmli, “Cleavage of structural proteins during the assembly of the head of bacteriophage T4,” Nature, vol. 227, no. 5259, pp. 680–685, 1970. View at Publisher · View at Google Scholar · View at Scopus
  25. R. Barderas, M. Villalba, and R. Rodríguez, “Che a 1: recombinant expression, purification and correspondence to the natural form,” International Archives of Allergy and Immunology, vol. 135, no. 4, pp. 284–292, 2004. View at Publisher · View at Google Scholar · View at Scopus