Cloning and Expression of Ama r 1, as a Novel Allergen of Amaranthus retroflexus Pollen

Table 1

Clinical attribute, skin prick test responses, and specific IgE values of patients reactive to recombinant Ama r 1.

Patients number	Age (years)/gender^1	Symptoms^2	Total IgE (IU/mL)	A. retroflexus pollen extract		rAma r 1-specific IgE
				Skin prick test^3	Specific IgE^4
1	38/M	A, R	152	8	1.80	0.95
2	32/F	A, R, L	185	12	2.10	1.23
3	21/F	A, R	162	8	0.98	0.80
4	38/F	A, R, L	224	12	1.89	1.10
5	23/M	A, R, L	132	10	1.10	0.84
6	29/F	A, R, L	166	12	1.95	0.98
7	41/M	A, R	175	9	0.92	0.81
8	32/F	A, R, L	305	15	2.21	1.15
9	22/M	A, R	159	11	1.12	0.87
10	35/F	A, L	178	10	0.97	0.78

 ¹M: male; F: female.
²A: allergic rhinitis; L: lung symptoms (breathlessness, tight chest, cough, and wheeze); R: rhinoconjunctivitis.
³The mean wheal areas are displayed in mm². Histamine diphosphate (10 mg/mL)—positive control; Glycerin—negative control.
⁴Determined in specific ELISA as OD (optical density) at 450 nm.