Simple Preparation of Pacific Cod Trypsin for Enzymatic Peptide Synthesis

Figure 3

SDS-PAGE and IEF of purified Pacific cod trypsin. (a) SDS-PAGE of purified Pacific cod trypsin. Lane 1 contains protein standards; egg albumin (molecular weight, 45.0 kDa), bovine pancreatic trypsinogen (24.0 kDa), bovine milk β-lactoglobulin (18.4 kDa), and egg white lysozyme (14.3 kDa). (b) IEF of purified Pacific cod trypsin. Lane 1 contains purified Pacific cod trypsin. Lane 2 contains IEF isoelectric point (pI) standards: phycocyanin (pI 4.45, 4.65, 4.75), β-lactoglobulin (5.1), bovine carbonic anhydrase (6.0), human carbonic anhydrase (6.5), equine myoglobin (6.8, 7.0), human hemoglobin (7.1), lentil lectin (7.8, 8.0, 8.23), and cytochrome c (9.6).

(a)

(b)