Polyamine-Induced Rapid Root Abscission in Azolla pinnata
Figure 3
Effect of pH on PA-induced root abscission in A. pinnata. Root abscission is represented as % of the initial number of roots. Assays were conducted in (a) neutral phosphate buffer in the presence of 2โmM spermine at pH 9 and pH 7 () and 2โmM spermine tetrahydrochloride (Spmยท4HCl) at pH 6.8 (), (b) neutral phosphate buffer in the presence of 2โmM spermidine at pH 8.3 () and pH 7 (), and (c) 10โmM potassium phosphate solution pH 4, 5, 6, 7, 8 (), 9 and 10 (). Hydrochloric acid was used to adjust the pH. In (c), since the results were identical, four representative data (pH 4, 6, 8, and 10) are shown for simplification. Standard error bars are only shown where larger than the symbols used.