Elucidation of the Rotavirus NSP4-Caveolin-1 and -Cholesterol Interactions Using Synthetic Peptides

Table 2

Binding affinities (𝐾_𝑑) for NSP4_112-140 + caveolin-1 peptides in the absence and presence of SUV model membranes^a.

Sample	𝐾𝑑 (nM) in absence of SUV	𝐾𝑑 (nM) in presence of SUV
Cy3-N-Cav2-20 + NSP4112-140	85±6	26±4
Cy3-N-Cav19-40 + NSP4112-140	40±10	37±7
Cy3-C-Cav161-178 + NSP4112-140	217±35	172±22

^aThe 𝐾_𝑑values were calculated as outlined in Materials and Methods and are presented as means ± SD,𝑛=4.