Metabolic Fate of the Increased Yeast Amino Acid Uptake Subsequent to Catabolite Derepression
Figure 2
Presence of glucose or galactose in the medium is essential to measure L-[14C]leucine (750 μM) oxidation in WT (M3750) yeast. Yeast was grown in 2% glucose (filled bars) or 2% galactose (hollow bars) and incubated in the assay medium with glucose, galactose (± carbohydrate, CHO), or the nonmetabolizable glucose analogue deoxyglucose (DOG) as described in experimental procedures. (Oxidation in yeast grown in glucose in the presence of DOG was not measured.) Results are means ± SEM for 3 different preparations measured in quadruplicate.
